Home > AT1 Study, H&E > Hematoxylin-Eosin Stain

Hematoxylin-Eosin Stain

Today I finished my H&E staining for the different regions of the brain. The staining protocol consists of about 25 different steps and takes about an hour to stain 24 slides. The hematoxylin dye is used to stain the nuclei of the cells purple and the eosin dye stains the cytoplasm around the nuclei a shade of pink. We are using the H&E stained brain slices to count the total number of neurons in the hypoglossal nucleus, which we can then compare to the total number of AT1 positive neurons (the immunohistochemistry stuff) to see  if there is a correlation. Like the AT1 groups, I used a control group, a dystrophic group, and lostaran treated dystrophic group to make the comparison. I have yet to gather data but in the mean time here are some pictures….

 

Blog Pic of 5-3 B4 10x

The AT1 Staining at 10x

                                        

Hematoxylin Eosin Stain 10x

The Hematoxylin-Eosin Staining at 10x

                               

AT1 stain 20x

AT1 at 20x

                                                 

Hematoxylin Eosin Stain 20x

H&E at 20x

                                                       

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Categories: AT1 Study, H&E
  1. evsociety09
    July 16, 2009 at 10:53 pm

    Mitch, it looks like things are going well and I am interested to see what your findings will show. I hope you can get your results soon so that we can see if there is a correlation or not. How long does it take to interpret you data? I assume it is not very straight forward and was wondering what you look for when collecting the data?

  2. Josh Doorn
    July 17, 2009 at 3:23 pm

    These images look very interesting. Do you have to count every small dot on the stain or just the big blotches or both? What exactly is the Hematoxylin-Eosin that you are staining with purple? Good Luck with the rest of the year!

  3. B. F. Pons
    July 18, 2009 at 7:31 pm

    Everything on the experimental side seems to be going well. I was wondering if you have had a chance to update your paper in between all the experiments that you have been conducting. It seemed like you had a good start but I would be definitely interested in reading the rest.

    Good Job and Good Luck

  4. karlgiu
    July 19, 2009 at 4:54 am

    You mentioned during your presentation that this is one part of the overall study? Can you offer the conclusions in a broader context of the research project? I suppose that this extends to the significance of your study. Could such a thing either appear on the poster or will it just be mentioned during the course of your poster presentation?

  5. usdneuro
    July 20, 2009 at 12:18 am

    What data do you expect to have for the poster session on the 27th? How has the project been going since your presentation?

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