Archive for the ‘H&E’ Category


July 20, 2009 3 comments

I finished my poster today and was surprised that it only took a few hours to put together. The abstract we wrote earlier came in handy for an intro and I actually kind of enjoyed making this…

U.Discover Poster

U.Discover Poster

Categories: AT1 Study, H&E

Hematoxylin-Eosin Stain

July 15, 2009 5 comments

Today I finished my H&E staining for the different regions of the brain. The staining protocol consists of about 25 different steps and takes about an hour to stain 24 slides. The hematoxylin dye is used to stain the nuclei of the cells purple and the eosin dye stains the cytoplasm around the nuclei a shade of pink. We are using the H&E stained brain slices to count the total number of neurons in the hypoglossal nucleus, which we can then compare to the total number of AT1 positive neurons (the immunohistochemistry stuff) to see  if there is a correlation. Like the AT1 groups, I used a control group, a dystrophic group, and lostaran treated dystrophic group to make the comparison. I have yet to gather data but in the mean time here are some pictures….


Blog Pic of 5-3 B4 10x

The AT1 Staining at 10x


Hematoxylin Eosin Stain 10x

The Hematoxylin-Eosin Staining at 10x


AT1 stain 20x

AT1 at 20x


Hematoxylin Eosin Stain 20x

H&E at 20x


Categories: AT1 Study, H&E